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主营:神经科学、干细胞以及再生医学研究试剂
℡ 4000-520-616
℡ 4000-520-616
Neuromics/Mu Opioid Receptor/P10104/50 ul Blocking Peptide @ 2mgs/ml
市  场 价:¥1900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$95.00
品      牌: Neuromics
公      司:Neuromics
公司分类:
Neuromics/Mu Opioid Receptor/P10104/50 ul Blocking Peptide @ 2mgs/ml
商品介绍

Type:GuineaPigIgG

 

Applications:ICC;IHC

E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssays

SpeciesReactivity:H;M;Pr;R

B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All

Format:AffinityPurified-liquid

 

Immunogen:NHQLENLEAETAPLP

 

Image of Mu Opioid Receptor or MOR staining of Rat DRGs

Threetypesofopioidreceptorshavebeencloned--mu,delta,andkappa.OpioidreceptorsareseventransmembraneG-proteincoupledreceptors.TheyshareahighdegreeofhomologyandaremostdivergentattheN-andC-termini.ActivationofmuopioidreceptorsleadstoadecreaseinneuronalexcitABIlity.

Image:MuOpioidReceptorstainingofRatDorsalHorn.CourtesyofDr.LouisGendron,UniversityofSherbrooke.

Protocol:
  1. Ratswere deeplyanesthetized withisoflurane andperfusedthroughtheaorticarchwith 100mlofheparin(75U/mlofheparinin0.9%saline)followedby100mlofamixtureof3.75%acroleinand2%PFAin0.1MPB,pH7.4,andthenby300mlof2%PFAinthesamebufferat45ml/min.mLumbar spinalcordwas removedandpostfixedin2%PFAin0.1MPBfor1hat4°C.Sections(50µmthick)werecutusingaVibratomeandprocessedforMORlabeling.

  2. Sectionswereincubatedin1%sodiumborohydridefor30minandextensivelyrinsedin0.1MPB.Theywerethencryoprotectedfor30mininasolutionconsistingof25%sucroseand3%glycerolin0.05MPBandsnapfrozenwithisopentane(–50°C)followedbyliquidnitrogen.

  3. Afterbeingrapidlythawedin0.1MPB,sectionswererinsedwithTBS 0.1M andpreincubatedfor1hatroomtemperaturein3%NGSdilutedinTBS.Theywerethenincubatedfor36hat4°CinMORantiserum diluted 1/500inTBScontaining0.5%NGS.SectionswerethenrinsedtwicewithTBSandincubatedfor1hatroomtemperaturewithbiotinylatedanti-guineapigantibody(1/400;VectorLaboratories).

  4. Followingthree10minwashesinTBS,sectionswereincubated30minwith VectastainEliteABC(VectorLaboratories).SectionswererinsedthreetimeswithTBSandperoxidasecomplexrevealedfor8minuteswithDABsubstrate(2.2mg/10ml+0.01%H2O2).

  5. Attheendofthisincubation,sectionswerewashedtwicewithTBS, mountedonmicroscopeslides,anddehydratedwithethanol.

MuOpioidReceptorCustomerPublications

品牌介绍

Neuromics公司提供一系列高品质新蛋白的抗体、神经干细胞及培养基、神经干细胞扩增及分化试剂盒、干细胞抗体、神经和前体细胞流式分型试剂、细胞凋亡研究试剂、荧光激活细胞分拣法试剂盒、细胞内部试剂盒、转染试剂、ELISA 缓冲稀释液与IHC-WB-ELISA 补充物等试剂。

Neuromics因为是由一批顶尖的学术和商业实验室挑选,试验和创新他们的 试剂来满足顾客的需要而使自己的表现尤为突出。Neuromics从 以上过程中获得的经验使得顾客****的实际应用和试剂独特的性能能够得以相容。它精通于提供试剂和为神经科学研究机构提供产品和服务。


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