Neuromics/Cas9 from S. pyogenes/RA22126/100 ul,Neuromics,,Neuromics,Neuromics INC,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Neuromics/Cas9 from S. pyogenes/RA22126/100 ul

产品编号：RA22126-100ul

市场价：¥5900.00

场地：美国(厂家直采)

产品分类：抗体类>一抗>功能性抗体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$295.00

品牌： Neuromics

公司：Neuromics

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商品介绍

Type:RabbitIgG

Applications:ICC;IF;WB

E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssays

SpeciesReactivity:NotApplicable

B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All

Format:AffinityPurified-liquid

ThediscoveryofCRISPR(ClusteredRegularlyInterspacedShortPalindromicRepeats)haschangedthefieldofgeneediting.TheserepeatedsequencesarefoundinbacterialgenomeswithshortDNAsequencesderivedfromviruseswhichhaveinfectedthebacteriainterspaced.ThesevirallyderivedsequencescanmakeshortRNAsequenceswhichcanhybridizewithspecificviralDNAandtargetanuclease,suchasCas9,totheviralsequence.So,ifthebacteriaareinfectedbythisvirusagain,Cas9canbedirectedtocleavethespecificviralsequenceandsoinactivatethevirus.BycarefuldesignoftheRNAsequencethesystemcanbeusedtospecificallycutDNAvirtuallyanywhere,includinginlivinghumanandothermammaliancells.Thisallowsinexpensivegeneeditingwithunprecedentedease,andmucheffortisgoingintorefiningtheCas9enzymesandtheirrelativesforuseinmammaliansystems.

Image:Hek293cellculturetransfectedwithaconstructincludingtheCterminal814-1372aminoacidsofStaphylococcuspyogenesCas9.ThecellswerestainedwithRA22126inredandalsowithachickenantibodytoS.pyogenesCas9ingreen.AcellexpressingCas9thereforeappearsyellow.MostHek293cellsarenottransfectedsoonlythenucleusofthesecellscanbevisualizedwiththeblueDNAstain.

SeveralvarietiesofCas9havebeenstudiedandthereappeartobeseveralotherrelatedenzymeswithsimilarpropertiesinbacteria.TwoCas9homologsincludeStreptococcuspyogenseandStaphylococcusaureus.Staphylococcusaureusissignificantlysmallerandsopresentslessproblemswhenpackagedintovectors.TheS.pyogenesproteinisratherlargeat1,368aminoacids,~160kDa,sothecorrespondingDNAisalsoratherlargeatabout4.2kb.Ourantibodyisanaffinitypurifiedpolyclonalraisedinrabbitagainstamixtureoftworecombinantconstructscorrespondingtoaminoacids1-608and814-1372ofCas9fromtheStreptococcuspyogenesandbindsboththeseproteinstransfectedintocellsandonwesternblots,andalsorecognizesthefulllengthprotein.ThehomologousregionoftheS.aureusCas9isnotcloselyrelatedinaminoacidsequenceand,asexpected,thisantibodydoesnotrecognizethatprotein.

Image:WesternblotanalysisofRA22126
Blotof100ng(lane1)and10ng(lane2)offulllengthCas9proteinfromStreptococcuspyogeneswasprobedwithRA22126at1:2,000dilution.ThisantibodyrecognizesfulllengthCas9proteinat160kDaandaproteolyticfragmentat130kDa.

品牌介绍

Neuromics公司提供一系列高品质新蛋白的抗体、神经干细胞及培养基、神经干细胞扩增及分化试剂盒、干细胞抗体、神经和前体细胞流式分型试剂、细胞凋亡研究试剂、荧光激活细胞分拣法试剂盒、细胞内部试剂盒、转染试剂、ELISA 缓冲稀释液与IHC-WB-ELISA 补充物等试剂。

Neuromics因为是由一批顶尖的学术和商业实验室挑选，试验和创新他们的试剂来满足顾客的需要而使自己的表现尤为突出。Neuromics从以上过程中获得的经验使得顾客****的实际应用和试剂独特的性能能够得以相容。它精通于提供试剂和为神经科学研究机构提供产品和服务。

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