Type:GoatIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,E.coli-derived,recombinanthumanMothersAgainstDPPhomolog1(rhSmad1;aa2-454).
SMAD1isamemberoftheMothersAgainstDpp(MAD)-relatedfamilyofproteins.Sofar,eightSMADshavebeenidentifiedandcanbedividedinto3subgroupsbasedontheirstructureandfunction;pathway-restricted,commonmediatorandinhibitory.SMAD1,5and8serveaspathway-restrictedSMADsforthebonemorphogeneticprotein(BMP)signalingpathway.
Smad1isphosphorylatedresultinginitsassociationwiththecommon-mediatorsubunit,Smad4.Thisheteromericcomplexthentranslocatesintothenucleustoexerttranscriptionalcomodulatoractivity.
Image:DetectionofSmad1.Lysatesfrom5x105humanHepG2,MDA-MB-468,andHT29cellswereresolvedbySDS-PAGE,transferredtoImmobilon-Pmembrane,andimmunoblottedwith0.5μg/mLgoatanti-Smad1asdescribedinProtocolsforImmunoblotting.A30secondexposuretofilmisshown.
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