Neuromics/TNF-alpha Human Sandwich ELISA Kit/ELH-TNFalpha-001/1 Plate Kit,Neuromics,,Neuromics,Neuromics INC,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Neuromics/TNF-alpha Human Sandwich ELISA Kit/ELH-TNFalpha-001/1 Plate Kit

产品编号：ELH-TNFalpha-001

市场价：¥7580.00

场地：美国(厂家直采)

产品分类：抗体类>一抗>功能性抗体>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$379.00

品牌： Neuromics

公司：Neuromics

公司分类：

商品介绍

Type:Kit

Applications:E

E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssays

SpeciesReactivity:H

B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All

Sensitive,SpecificandCostEffective

TNF-alpha(tumornecrosisfactor-a)issecretedbymacrophages,monocytes,neutrophils,T-cells,NK-cellsfollowingtheirstimulationbybacteriallipopolysaccharides.HumanTNF-alphaisanon-glycosylatedproteinof17.5kDaandalengthof157aminoacid.TNF-alphashowsawidespectrumofBIOLOGicalactivities.Itcausescytolysisandcytostasisofmanytumorcellinesinvitro.WithinhoursafterinjectionTNF-alphaleadstothedestructionofsmallbloodvesselswithinmalignanttumors.TNF-alphaalsoenhancesphagocytosisandcytotoxicityinneutrophilicgranulocytesandalsomodulatestheexpressionofmanyotherproteins.Ingeneral,TNF-alphaandTNF-betadisplaysimilarspectraofbiologicalactivitiesinvitrosystemsalthoughTNF-betaisoftenlesspotentordisplaysapparentpartialagoNISTactivity.

Figure:TNF-alphastandardcurve

SuggestedApplication:QuantitativeProteinDetection;EstablishingNormalRange;ValidationofAntibodyArrayResults.

KitComponents:

· Pre-Coated96-WellStripMicroplate

· WashBuffer

· StopSolution

· AssayDiluent(s)

· LyophilizedStandard

· BiotinylatedDetectionAntibody

· Streptavidin-ConjugatedHRP

· TMBOne-StepSubstrate

OtherMaterialsRequired:

· Distilledordeionizedwater

· PrecisionPipettestodeliver2μlto1mlvolumes

· Adjustable1-25mlpipettesforreagentpreparation

· 100mland1litergraduatedcylinders

· Tubestopreparestandardandsampledilutions

· Absorbentpaper

· Microplatereadercapableofmeasuringabsorbanceat450nm

· Log-loggraphpaperorcomputerandsoftwareforELISAdataanalysis

ProtocolOutline:

1.Prepareallreagents,samplesandstandardsasinstructedinthemanual.

2.Add100μlofstandardorsampletoeachwell.Incubate2.5hatRTorO/Nat4°C.

3.Add100μlofpreparedbiotinantibodytoeachwell.Incubate1hatRT.

4.Add100μlofpreparedStreptavidinsolutiontoeachwell.Incubate45minatRT.

5.Add100μlofTMBOne-StepSubstrateReagenttoeachwell.Incubate30minatRT.

6.Add50μlofStopSolutiontoeachwell.Readat450nmimmediately.

Figure:BoxplotoftheserumIL-6levelintheRApatientsandcontrolaccordingtotheIL-6(−174G/C)promoterpolymorphismtype.http://dx.doi.org/10.1016/j.ejr.2012.11.002

品牌介绍

Neuromics公司提供一系列高品质新蛋白的抗体、神经干细胞及培养基、神经干细胞扩增及分化试剂盒、干细胞抗体、神经和前体细胞流式分型试剂、细胞凋亡研究试剂、荧光激活细胞分拣法试剂盒、细胞内部试剂盒、转染试剂、ELISA 缓冲稀释液与IHC-WB-ELISA 补充物等试剂。

Neuromics因为是由一批顶尖的学术和商业实验室挑选，试验和创新他们的试剂来满足顾客的需要而使自己的表现尤为突出。Neuromics从以上过程中获得的经验使得顾客****的实际应用和试剂独特的性能能够得以相容。它精通于提供试剂和为神经科学研究机构提供产品和服务。

公司介绍

产品分类

更多...