Type:RabbitIgG
Applications:IF;IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Aminoacids185-197ofHumanVDAC1/Porin1.
VDAC(alsoknownasVoltage-dependentanion-selectivechannelprotein1,Outermitochondrialmembraneproteinporin1,Plasmalemmalporin,Porin31HL)isanoutermembranemitochondrialprotein.TheVDACproteinsare~30-33kDa(someisoformsarelarger-seebelow).TheVDACproteinsarethoughttoformaqueouschannels,orpores,throughwhichadeninenucleotidescrosstheoutermito-chondrialmembrane.VDACshavebeenimplicatedintheformationofthemitochondrialpermeABIlitytransitionporecomplexinapoptoticcells.Thiscomplex,formedbyVDAC,adeninenucleotidetranslocator(ANT),andcyclophilinD(CypD),isthoughttoallowthemitochondriatoundergometabolicuncouplingandirreversIBLemorphologicchangesthatultimatelydestroythemitochondriaduringapoptosis.VDACsarehighlyexpressedinheart,liverandskeletalmuscle,whereconcentrationsofmitochondriaareattheirhighest.ThisantibodycanbeusedasaloADIngcontrolwithwholecelllysatesandtotalmitochondrialpreparations.
Image:VDAC1stainingofHumanLiver:Formalin-FixedParaffin-Embedded(FFPE).
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