Type:GoatIgG
Applications:E;ICC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,E.coli-derived,recombinanthumanrhRUNX2protein(aa219-404).
Runt-relatedtranscriptionfactor2(RUNX2),alsoknowasCBFA1, isa56kDaDNA-bindingproteinthatbelongstotheRunt-relatedfamilyoftranscriptionfactors.Itissynthesizedasa521aminoacid(aa)proteinwithmultipledomains.FromtheN-terminus,thereisa19aaactivationdomain(AD1),a41aaglutamine/alanine(Q/A)repeatsegment(AD2),a128aaRunt/DNA-bindingregion,a9aaNLSanda285aaProSerThr(PST)-richsequencethatisneededforgeneactivation.Twoisoformsexist,onewithatruncatedAD1domainandanotherwitha22aadeletionintheAD3sectionofPST-richsequence.HumanCBFA1is98%aaidenticaltomouseCBFA1.
Image:DetectionofCBFA1/RUNX2inMC-3T3cellsbyICC.
RUNX2isMarkerforMesenchymalStemCells/OsteoblastDifferentiation.
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