IGF-Ireceptorisadisulfide-linkedheterotetramerictransmembraneproteinconsistingoftwoalphaandtwobetasubunits.BoththealphaandbetasubunitsareencodedwithinasinglereceptorprecursorCDNA.Theproreceptorpolypeptideisproteolyticallycleavedanddisulfide-linkedtoyieldthematureheterotetramericreceptor.ThealphasubunitofIGF-Ireceptorisextracellularwhilethebetasubunithasanextracellulardomain,atransmembranedomainandacytoplasmictyrosinekinasedomain.TheIGF-Ireceptorishighlyexpressedinallcelltypesandtissues.
Images:(1)LysateswerepreparedfromNTERA-2humanembryonalcarcinomacells,SK-MEL-28humanmalignantmelanomacells,andG-361humanmalignantmelanomacellsinnon-reducingsamplebuffer.ThelysateswereresolvedbySDS-PAGEandtransferredtoanImmobilon-Pmembrane.Theblotwasdevelopedwith1μg/mLmonoclonalanti-humanIGF-IRantibodyandWesternGloChemiluminescentDetectionSubstrate.(2) IGF-IR(atadilutionof25µg/mL) stainingof paraffin-embeddedhumanskintissue. Tissue stainedwith anti-mouseHRP-DAB(Brown)andcounterstainedwithhaematoxylin(blue).
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