Type:GoatIgG
Applications:IHC;WB;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,E.coli-derived,recombinanthumaninsulin-likegrowthfactorII(rhIGF-II).
Insulin-likegrowthfactorI(alsoknownassomatomedinCandsomatomedinA)andinsulin-likegrowthfactorII(multiplicationstimulatingactivityorMSA)belongtothefamilyofinsulin-likegrowthfactorsthatarestructurallyhomologoustoproinsulin.MatureIGF-IandIGF-IIshareapproximately70%sequenceidentity.BothIGF-IandIGF-IIareexpressedinmanytissuesandcelltypesandmayhaveautocrine,paracrineandendocrinefunctions.MatureIGF-IandIGF-IIarehighlyconserved(100%identitybetweenhuman,bovineandporcineproteins)andexhibitcross-speciesactivity.IGF-IIisapotentmitogenicgrowthfactor.
IGF-IIbindingissignificantlyincreasedinthediabetickidneyandcouldplayaroleindiabeticneuropathy.Italsoappearstobeinvolvedsignificantlyintheprogressionofmanytumors
Image:IGF-IIstainingofParaffin-embeddedtissuesectionsofhumanplacenta(chorionicvilli).Tissuewas HRP-AECCell (red)andcounterstainedwithHaematoxylin(blue).
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