Type:GoatIgG
Applications:IHC;WB;FC;NB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Purified,NS0-derived,recombinanthumanToll-likereceptor4(rhTLR4;Glu24-Lys631;Accession#O00206)extracellulardomain.
Toll-LikeReceptor4(TLR4)hasbeenidentifiednexttoMD2andCD14asareceptorthatiscentraltotheinnateimmuneresponsetolipopolysaccharides(LPS)ofGramnegativebacteria.TLR4contributestotheinitiationofCNSneuroimmuneactivationafternervetransection.TLR4ispartofanearly,specific,innateCNS/microglialresponseandmayberelevantinthepreventionandtreatmentofneuropathicpainsyndromes.TLR4isweaklyexpressedbyrestingcells,butisupregulatedfollowingstimulationwithLPS.
Image:Toll-LikeReceptor4(TLR4orCD284)stainingofimmersionfixedparaffin-embeddedsectionsofhumanprostateat10 µg/mLovernightat4 °C.Beforeincubationwiththeprimaryantibodytissuewassubjectedtoheat-inducedepitoperetrievalusingAntigenRetrievalReagent. TissuewasstainedusingtheAnti-GoatHRP-DAB.(brown)andcounterstainedwithhematoxylin(blue).
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