Type:MouseIgG
Applications:ICC;IF;IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:FulllengthrecombinanthumanproteinexpressedinandpurifiedfromE.coli
c-FosispartoftheFosgenefamily;whichconsistsof4members:FOS,FOSb,FOSL1andFOSL2.FosfamilygenesareassociatedwithJUNproteinsandotherbasicleucineipper(bZIP)domainproteinstocreateavarietyofAP-1(activatorprotein-1)complexes.c-Fosisconsideredtobeanimmediate-earlygenebecauseexpressionisnormallylowbutrapidlyandtransientlyinresponsetoawidearrayofstimuliincludingserum,growthfactors,tumorpromoters,cytokines,andUVrADIation.Itplaysanimportantroleinmanycellularfunctions,andisover-expressedinvarietyofcancers.
Left: MO22162staining(green)inHeLacells,whichweretreatedwithserum-starvationfor36hours,followedby2hours,20%FBSstimulation(bottompanel),orPBStreatment(toppanel).Greenc-Fosstainingonlylocalizesinthenucleiofstimulatedcells,butnotinun-stimulatedcells.Cellsarecounter-stainedwithourchickenpolyclonalantibodyagainstvimentin,CH22108inred).BlueshowsDAPIstainingofnucleus．Middle: Mousebrainsection(45μM;fixedbytranscardialperfusionwith4%paraformaldehyde)labeledwithMO22162usingastandardHRP-DAB(horseradishperoxidase-3,3’-diaminobenzidine)stainingtechnique.Cellsexpressingc-Fosshowdarkcolorinnucleus. Right: MousecorticalsectionlabeledwithMO22162(red)andourrabbitpolyclonalantiFox3/NeuN(RA22113)antibody(green)usingimmuno-fluorescentconfocal-microscopy.Neuronspositiveforc-FosandFox3/NeuNappeartobeyellow.InsetshowsanenlargedimageofMCA-2H2staining.NucleiarelabeledwithDapi(blue)
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