Type:SheepIgG
Applications:WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
c-JunisacomponentofthetranscriptionfactorAP-1,whichbindsandactivatestranscriptionatTRE/AP-1elements.Thetranscriptionalactivityofc-JunisregulatedbyphosphorylationatSer63andSer73(1,2).Extracellularsignalsincludinggrowthfactors,transformingoncoproteinsandUVirrADIationstimulatephosphorylationofc-JunatSer63/73andactivatec-Jun-dependenttranscription.MutationofSer63/73rendersc-Junnonresponsivetomitogenicandstress-inducedsignalingpathways.TheMAPkinasehomologueSAPK/JNKbindstotheamino-terminalregionofc-Junandphosphorylatesc-JunatSer63/73.Inaddition,theactivityofSAPK/JNKisstimulatedbythesamesignalsthatactivatec-Jun(3,4).
c-Junalso playsaroleinNeuropathicPain.
Image:Detectionofc-JunwithSP15102.LysatesfromhumanHeLa(1),K562(2),andMDA-MB-468(3)cellswereresolvedbySDS-PAGE,transferredtoImmobilon-Pmembrane,andimmunoblottedwith0.5ug/mLsheepanti-c-jun. A1minuteexposuretofilmisshown.Bandforc-Junis~40KDa.
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