Type:GoatIgG
Applications:IHC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Syntheticpeptide:SQRPSRCSNPTPGP,fromtheCTerminusoftheproteinsequenceaccordingtoNP_054728.2;NP_001107849.1.
FOXP3isamemberoftheforkhead/winged-helixfamilyoftranscriptionalregulatorsandishighlyconservedacrossmammals.FOXP3isessentialfornormalimmunehomeostasis.FOXP3isstablyandconstitutivelyexpressedatahighlevelinCD25+CD4positiveregulatoryTcells,alowlevelinCD4-positive/CD25-negativecells,andisabsentinCD4-negative/CD8-positiveTcells.FOXP3maybeamasterregulatorygeneandamorespecificMarkerofregulatoryTcellsthanotherTcells.
DefectsinFOXP3arethecauseofimmunodeficiencypolyendocrinopathy,enteropathy,X-linkedsyndrome(IPEX)[MIM:304790];alsoknownasX-linkedautoimmunity-immunodeficiencysyndrome.IPEXischaracterizedbyneonatalonsetinsulin-dependentdiabetesmellitus,infections,secretorydiarrhea,trombocytopenia,anemiaandeczema.Itisusuallylethalininfancy.
Image:FOXP3stainingofstainingofparaffinembeddedHumanTonsil(dilution:1µg/ml).MicrowavedantigenretrievalwithTris/EDTAbufferpH9,HRP-staining.A)Nuclearstainingofscatteredcellsintheinterfolliculararea.B)Highmagnificationofpositivecells.Protocolondata-sheet.
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