Type:MouseIgG
Applications:FC
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:Clone#:42704
ThehumanC-X-CchemokineIL-8isapotentneutrophilchemotacticandactivatingfactor(1).TwodistinctcellsurfacereceptorscaninteractwiththeIL-8molecule.ThesetwostructuresareknownasIL-8RA(typeIorCXCR-1)andIL-8RB(typeIIorCXCR-2)(2,3).Thetworeceptorsshare77%aminoacidhomologyandarebothmembersoftherhodopsinsuperfamilyofGprotein-linkedreceptorsthatspanthecellmembranewithsevenhydrophobictransmembranedomains.OfthetwoIL-8receptors,onlyIL-8RAexclusivelybindsIL-8(4).AthirdIL-8bindingstructure,knownastheDuffybloodgroupantigen,hasbeenidentifiedonredbloodcells(5).IL-8RAexpressionhasbeendocumentedonneutrophils,monocytesandasmallpopulationofTcells(2).
References
1.Oppenheim,J.J.(1991)Annu.Rev.Immunol.9:617.
2.Holmes,W.E.etal.(1991)Science253:1278.
3.Murphy,P.M.andH.L.Tiffany(1991)
Science253:1280.
4.LaRosa,G.J.etal.(1992)J.Biol.Chem.267:25402.
5.Horuk,R.etal.(1993)Biochemisry32:5733
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