CXCR-4(fusinorLESTR)isaG-proteinlinkedseventransmembranespanningchemokinereceptorthatbindsstromalcellderivedfactor-1(SDF-1)(1,2).Additionally,CXCR-4hasbeenfoundtoactasaco-factorfortheT-celltropicHIV-1and-2virusentryintocells(3,4).Amonoclonalantibody(clone12G5)totheCXCR-4structurewasdevelopedbyEndersetal.(4)andshownbyflowcytometryapplicationstoreactwithCXCR-4expressedonavarietyofhumancelllines,includingSup-T1,Hut-78,Molt4,CEMss,DaudiandHela,aswellaswithperipheralbloodlymphocytes(4).Clone12G5canneutralizeinfectionandinhibitsyncytiumformationinducedbytheHIVvirus(4).Theepitopeforthe12G5monoclonalantibodymapstoaregionoftheCXCR-4receptorthatincludesextracellularloops1and2,withthefirstextracellularloopplayingamorecriticalroleintheepitopestructure(5).R&DSystemshasdevelopedaseriesofmonoclonalantibodiesthatspecificallyreactwiththehumanCXCR-4structureontransfectedcellsaswellastheCXCR-4antigenonbothstimulatedandunstimulatedperipheralbloodlymphocytes.Clone44717.111appearstoreactpreferentiallywiththesecondexternalloopregionoftheCXCR-4receptor.CXCR4CustomerPublications.
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