TheInterleukin1receptorfamily(IL-1R)comprisesatleastninemembersincludingIL-1R1,IL-1R2(IL-1RII),IL-1R3(IL-1RAcP),IL-1R4(ST2/T1),IL-1R5(IL-18Ralpha/IL-1Rrp),IL-1R6(IL-1Rrp2/IL-1RL2),IL-1R7(AcPL/IL-18Rb),IL-1R8(TIGIRR-2)andIL-1R9(TIGIRR-1).Allfamilymemberspossessthreeimmunoglobulin(Ig)-likedomainsintheirextracellularregion.MostmembersalsohaveanintracellularTIR(Toll-likereceptor/IL-1receptor)signalingdomainthatisalsoconservedintheToll-likereceptorfamily.AsubtypeoftheIL-1Rfamily,SIGIRR(singleIgdomain-containingIL-1R-relatedmolecule)whichdiffersfromtheotherninemembersbyhavingonlyoneIgdomain,hasalsobeenidentified.SeeIL-18RalphaforIL-1R5andIL-18RbetaforIL-1R7.
Image:IL-1betawasdetectedinLPS-stimulatedratsplenocytes.Cellswerestainedusinganti-goatIgGsecondaryantibody(red)andcounterstainedwithFluoroNissl™*Green.
Figure1:RatIL-1βstimulates3H-thymidineincorporationbymurineT-helperD10.G4.1cellsinadose-dependentmanner(Symons,J.A.etal.,1987,inLymphokinesandInterferons,APracticalApproach,IRLPress,M.J.Clemens,A.G.MorrisandA.J.H.Gearing,eds.p.272).TheED50forthiseffectistypically1-3ng/mL.
Figure2:Approximately0.5-2.0μg/mLoftheantibodywillneutralize50%ofthebioactivitydueto10ng/mLofratIL-1β.TheND50forthislotofanti-ratIL-1βantibodywasdeterminedtobeapproximately0.5-2μg/mLinthepresenceof10ng/mLofrrIL-1β,usingthemurineT-helpercellline,D10.G4.1.Thespecificconditionsaredescribedinthefigurelegends.
Neuromics公司提供一系列高品质新蛋白的抗体、神经干细胞及培养基、神经干细胞扩增及分化试剂盒、干细胞抗体、神经和前体细胞流式分型试剂、细胞凋亡研究试剂、荧光激活细胞分拣法试剂盒、细胞内部试剂盒、转染试剂、ELISA 缓冲稀释液与IHC-WB-ELISA 补充物等试剂。
Neuromics因为是由一批顶尖的学术和商业实验室挑选,试验和创新他们的 试剂来满足顾客的需要而使自己的表现尤为突出。Neuromics从 以上过程中获得的经验使得顾客****的实际应用和试剂独特的性能能够得以相容。它精通于提供试剂和为神经科学研究机构提供产品和服务。