Type:MouseIgG
Applications:E;FC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:HybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromamouseimmunizedwithpurified,E.coli-derived,recombinanthumanIL-17(rhIL-17;aa20-155;Accession#Q16552).
Interleukin-17(IL-17)isapro-inflammatorycytokinesecretedbyactivatedTcells.ItistheprototypememberoftheIL-17familythatalsoincludesIL-17B,C,D,E,andF.
IT exhibitsmultipleBIOLOGicalactivitiesonavarietyofcellsincluding:theinductionofIL6,IL8andGCSFproductioninfibroblasts;theenhancementofsurfaceexpressionofICAM1infibroblasts;activationofNFkappaBandcostimulationofTcellproliferation.IL17isanapproximately16kDapolypeptideof136aminoacids.TheprecursorformofIL17consistsof155aminoacids.TogeneratethematureIL17(136aminoacids),theprecursorcleavesa19aminoacidsignalpeptide.HumanIL17showsapproximately62.5%aminoacidhomologytomouseIL17and58%aminoacidhomologytoratIL17.
Image:Control(lightgreyhistogram)orPMAplusCa2+ionomycin-activated(darkgreyhistogram)humanPBMCswerestainedwithanti-IL-17orisotypecontrol(openhistogram)followedbyAPC-conjugatedanti-mouseantibody.
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