Type:MouseIgG
Applications:WB;FC;E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:ProteinGPurified-liquid
Immunogen:HybridomaresultingfromthefusionofamousemyelomawithBcellsobtainedfromamouseimmunizedwithpurified,E.coli-derived,recombinanthumanIL-17E(rhIL-17E;aa33-177;Accession#Q9H293).
Recently,anumberofcytokinesbelongingtotheinterleukinIL17familyhavebeenidentified.ThesearetermedasIL17B,IL17CandIL17E.IL17isapotentproinflammatorycytokinethatplaysrolesinanumberofdiseasesincludingrheumatoidarthritis,multiplesclerosis,andpromotionoftumorgrowth.IL17B,C,andElikeIL17areabletoinduceproinflammatoryresponses.However,theydonotbindtotheIL17receptorsuggestingthatadditionalIL17Rrelatedreceptormightexist.ReceptorforIL17BandIL17EhasbeenindependentlyisolatedandhavebeendesignatedasEV127(inmouse)andIL17RH1(inhuman),respectively.IL17EinducesactivationofNFkBpathwayandlikeIL17alsoinducesproductionofIL8.
Image:PC-3cellswerestainedwithanti-IL-17(filledhistogram)orisotypecontrolantibody(openhistogram)followedbyAPC-conjugatedanti-mouseantibody).
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