Type:MouseIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AscitesFluid-liquid
Immunogen:Epitopefor?-Synucleinisintheregion61-95whichcorrespondtothe"Non-AmyloidbetaComponentofAlzheimer"sdiseaseamyloid"
Alphasynucleinisamemberofthesynucleinfamily,theothertwoproteinsbeingbetaandgammasynuclein.AlphasynucleinwasoriginallyisolatedasamajorsynapticvesicleassociatedproteinfromtheelectricorganofthefishTorpedo(1).Directhomologuesofalpha-synucleinarefoundinallvertebrates.Laterworkconnectedalpha-synucleinwithhumanbrainpathology,whenaproteinoriginallyidentifiedasacomponentofNAC,the"Non-AmyloidbetaComponentofAlzheimer"sdiseaseamyloid"provedtobeapeptidederivedfromalpha-synuclein(2).
Image:Mixedneuron-glialculturesstainedwithalpha-synuclein (red)andchickenpolylclonalantibodytoMAP2 (green).Thealpha-synucleinantibodystainsvesicularstructurestheperikaryaandprocessesoftheneuronsinthis Notethatsomeoftheneuronalperikaryacontainmuchmorealpha-synucleinthanothers.ThebluechannelshowsthelocalizationofDNA. Inset:Blotsofantibodyagainst recombinantalpha-synuclein(leftlane)andcrudeextractofmousebrain(rightlane).ProtocolonData-sheet.
Thealpha-synucleinproteinisthereforesometimesknownasNACprecursororNACP.Furtherworkshowedthatalpha-synucleinisamajorcomponentoftheLewybodiesofParkinson"sdiseaseandpointmutationsofalpha-synucleinprovedtobecausativeofsomeformsoffamilialParkinson"sdisease(3,4,5).However,despitebeingdiscoveredasascomponentofamyloidpreparations,alpha-synucleinisapparentlynotamajorcomponentofthesenileplaquesofAlzheimer"sdisease(6).EarlyonsetParkinson"sdiseasemaybecausedbyaduplicationortriplicationofoneofthealphasynucleingenes(7,8).Alpha-synucleinisalsofoundintheLewybodiesofpatientswithdiffuseLewybodydiseaseandinclusionsinglialcellsinthebrainsofpatientswithmultiplesystematrophy(MSA)andamyotrophiclateralsclerosis(ALS).Alpha-synucleinisheavilyexpressedinbrainandappearstobelocalizedprimarilytopresynapticregions,thoughnotwithatypicalsynapticvesicledistributionpattern.Thesynucleinproteinsappeartohavelittle3Dstructureinsolution,andprobablybelongtothefamilyof"intrinsicallyunstructuredproteins"whichonlyadoptawell-definedconformationwhenboundtootherproteinsormembranelipids(9).Anexcellentrecentreviewoftheroleofalpha-synucleininhealthanddiseasewasrecentlypublishedbyMarkCookson(10).
Neuromics公司提供一系列高品质新蛋白的抗体、神经干细胞及培养基、神经干细胞扩增及分化试剂盒、干细胞抗体、神经和前体细胞流式分型试剂、细胞凋亡研究试剂、荧光激活细胞分拣法试剂盒、细胞内部试剂盒、转染试剂、ELISA 缓冲稀释液与IHC-WB-ELISA 补充物等试剂。
Neuromics因为是由一批顶尖的学术和商业实验室挑选，试验和创新他们的 试剂来满足顾客的需要而使自己的表现尤为突出。Neuromics从 以上过程中获得的经验使得顾客****的实际应用和试剂独特的性能能够得以相容。它精通于提供试剂和为神经科学研究机构提供产品和服务。
本网站将在规定时间内给予删除等相关处理。
