Type:ChickenIgY
Applications:IHC;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:H;M;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:liquid
Immunogen:RecombinantvimentinpurifiedfromE.Coli
Humanvimentinisa53,521daltonprotein(466aminoacids)expressedinmanycelltypesofmesodermalorigin,andisoneoftheintermediatefilamenttypesthatformtheCytoskeleton.Intheembryonicnervoussystem,however,vimentinisexpressedbymigratingneuralcrestcellsformingthePNS,andbyneuralstemcellsoftheCNS.Insomeformsofglioblastoma,vimentinisre-expressedbythetumorcellsandservesasageneralindicatoroftheirundifferentiatedstate.
ImmunohistochemicalstainingforVimentinisalsocharacteristicofsarcomas(ofneural,muscleandfibroblastorigin)comparedtocarcinomaswhicharegenerallynegative.Melanomas,lymphomasandvasculartumorsmayallstainforVimentin.Vimentinantibodiesarethusofvalueinthedifferentialdiagnosisofundifferentiatedneoplasmsandmalignanttumors.
Image:HumanHeLacellsstainedwithVimentinandour39C7nuclearporecomplexMarker.
MoreLinks:
VimentinAntibody-Cat#:CH23010 Neuronal-GlialMarkers-Astrocytes,Glia,Microglia,Olidogodendrocytes,ProgenitorsandSchwannCellMarkers | Neurotrophins-Neuron/GlialMarkerProteins CancerResearchAntibodies CancerResearchProteins | StemCellResearchReagents PrimaryNeuronsandAstrocytes-Primaryhuman,ratandmouseneuronsandastrocytes |
![Picture[3]](images/neuromics/2017/A8x872ex66x1.jpg)
Image:hN2PrimaryNeuronsstainedwithourchickenpolyclonalantibodytoVimentin,inred.IslandsofHn2cellsformafter4daysincultureformingbeautifulflowerlikestructures.Vimentinisawellestablishedmarkerofearlydifferentiatingneuronallineagecells.Takenwitha10Xobjectivelens.BluestainingisthenuclearDNA.Protocolsondata-sheet.
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