PGP9.5,Park5 orUCHL1 isasolublecytoplasmicproteinwithamolecularweightofapproximately25,000kD.Itispresentinneuronsandincellsofthediffuseneuroendocrinesystem.ItisanexcellentMarkerforneuronsoftheCNSandPNS.
Image:Mixedratneuron/glialculturesstainedwithPGP9.5/UCHL1(green).BlueisaDNAstain.NotethattheUCHL1stainsneuronsstronglyandspecifically,andthatthestainingisconcentratedinthecellbodies,thoughsomedoesextendintothedendritesalso.Surroundingglialcellsarenotstainedwiththisantibody,thoughmanyarepresentasvisualizedusingtheDNAstain.Inset:BlotofwholebovinebrainextractstainedwithPGP9.5/UCHL1showingastrongandcleanbandatabout24kDa.ProtocolonData-sheet.
Thegreatabundanceofthisproteininneuronsmeansthatitisreleasedfromneuronsinlargeamountsfollowinginjuryordegenerationand isdetected inCSFandotherbodilyfluids.Itis usedasa bio-markerforParkinson"sDisease(PD),Alzheimer"sDisease(AD),Axonaldystrophiesand TraumaticBrainInjury(TBI).
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