Type:MouseIgG
Applications:IF;WB
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:B;H;M;P;R
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllFormat:AffinityPurified-liquid
Immunogen:Actinpreparationderivedfrombovinebrain.
Actinisoneofthemostabundantandhighlyconservedproteinsofeukaryotes.Mammalianactinsaretheproductofsixdifferentgeneswithdifferingdistributionpatternsincelltypesandintissues.Themolecularweightofallsixproteinsis42kDa,andoneormoreactinsisfoundinessentiallyeverytypeofcrudecellularandtissueextract.Actinisregardedasa"housekeeping"proteinwhichisgenerallynotalteredmuchinexpressionasaresultofexperimentalmanipulations.Soquantitationoftheactinbandonthewesternblots isusedasastandardagainstwhichthebanddensityofotherproteinscanbecompared.Glyceraldehyde3phosphatedehydrogenase(GAPDH)isanother"housekeeping"proteinusedinthisway.Sinceourantibodybindsallsixisotypesverystronglyonwesternblots,itisaveryeffectiveblottingstandard.Italsoworksinimmunocytochemicalexperiments,bindingstonglyandcleanlytofilopodia,membranerufflesandstressfibers,allknowntoberichinactin
Image:WesternblotofcrudeextractofthehumancarcinomaHeLacellline.Lane15wasprobedwithActin,ourmonoclonalantibodytoallsixactinisotypes,givingastrongcleanbandatanSDS-PAGEmolecularweightof42kDa.lane16wasprobedwithourantibodyto14-3-3η(14-3-3eta),whichhasanSDS-PAGEmolecularweightof28kDa.Lanes17wasprobedwithourmonoclonalantibodytovimentin.ProtocolonDatasheet.
ActinisalsoaMarkerforautoimmunediseasesofthegutandcorrelatestointestinaldamage.Itisalsoassociatedwithcertainformsofhepatitis.
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