NogginisasecretedhomodimericglycoproteinthatisanantagoNISTofbonemorphogeneticproteins(BMPs).1,2HumanNogginCDNAencodesa232aminoacid(aa)precursorprotein;cleavageofa19aasignalpeptidegeneratesthe213aamatureproteinwhichcontainsanN-terminalacidicregion,acentralbasicheparin-bindingsegmentandaC-terminalcysteine-knotstructure.2SecretedNogginprobablyremainsclosetothecellsurfaceduetoitsbindingofheparin-containingproteoglycans.3Nogginisveryhighlyconservedamongvertebrates,suchthatmaturehumanNogginshares99%,99%,98%,97%and89%aasequenceidentitywithmouse,rat,bovine,equineandchickNoggin,respectively.NogginbindssomeBMPssuchasBMP-4withhighaffinityandotherssuchasBMP-7withloweraffinity,antagonizingBMPbioactivitiesbyblockingepitopesonBMPsthatareneededforbindingtobothtypeIandtypeIIreceptors.2,4Duringembryogenesis,NogginantagonizesspecificBMPsatdefinedtimesduringneuraltube,somiteandcardiomyocytegrowthandpatterning.5-7Duringskeletaldevelopment,Nogginpreventschondrocytehyperplasia,thusallowingproperformationofjoints.4Mutationswithinthecysteine-knotregionofhumanNogginarelinkedtomultipletypesofskeletaldysplasiasthatresultinapicaljointfusions.8Nogginisexpressedindefinedareasoftheadultcentralnervoussystemandperipheraltissuessuchaslung,skeletalmuscleandskin.1Duringcultureofhumanembryonicstemcells(hESC)withoutfeederlayersorconditionedmedium,butwithadditionofFGFbasic,additionofNoggintoantagonizeBMPactivityallowshESCtomaintaintheirundifferentiated,pluripotentstate.9,10
Neuromics公司提供一系列高品质新蛋白的抗体、神经干细胞及培养基、神经干细胞扩增及分化试剂盒、干细胞抗体、神经和前体细胞流式分型试剂、细胞凋亡研究试剂、荧光激活细胞分拣法试剂盒、细胞内部试剂盒、转染试剂、ELISA 缓冲稀释液与IHC-WB-ELISA 补充物等试剂。
Neuromics因为是由一批顶尖的学术和商业实验室挑选,试验和创新他们的 试剂来满足顾客的需要而使自己的表现尤为突出。Neuromics从 以上过程中获得的经验使得顾客****的实际应用和试剂独特的性能能够得以相容。它精通于提供试剂和为神经科学研究机构提供产品和服务。